Transformation Lab

In this lab we were creating bacteria and looking at what and where the bacteria grew better.


 Observations:

The LB/Amp positive was fluorescent green and did not glow but however it did contain many specks. Bacteria did grow a lot. It also contained antibiotics. Therefore this shows that bacteria even glows in the presence of antibiotics.



The Lb/Amp/ara positive contained arabinose. Arabinose activates sugar in some gene. Bacteria has grown in it but not as much as in the Lb/amp. This shows that bacteria grows better without the presence of antibiotics. And it glowed and was very florescent green.



The Lb negative did not grow any bacteria and only turned green.



The Lb/amp negative only had some condensation going on.


And this is what they look like all together.




 Results-
      The traits form colonies and their shape and color changed too.

Reviewing-

•       The plate that I would expect to see bacteria like the original non-transformed E. coli colonies would be the LB because in this plate, hopefully the bacteria would grow more because it is more like the original. 
•        If there was any genetically transformed bacterial cells they would be most likely in the LB/Amp/Ara because it would take DNA from the amp in order to live.
•       The plates that should be compared to determine if there is any genetic transformation occurring would be the PGLO and the positive PGLO in order to tell if the plasmid is transformed.
•       The meaning of a control plate is that you could control it. Like for example the ones in this experiment are the negative plates and the LB plates. 

This lab was very interesting and I learned many stuff from it.